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ethidium homodimer-1 (ethd-1)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ethidium homodimer-1 (ethd-1)
    Ethidium Homodimer 1 (Ethd 1), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethd+1/ethidium+homodimer+1/pm40662251-207-6-12
    Average 90 stars, based on 1 article reviews
    ethidium homodimer-1 (ethd-1) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Ethidium Homodimer Assay:

    Article Title: Electrospun Poly(carbonate-urea-urethane)s Nonwovens with Shape-Memory Properties as a Potential Biomaterial.
    Article Snippet: .. The assay was performed using calcein AM (2 μM, C1430 Life technologies) and ethidium homodimer-1, EthD-1 (E1169, 4 μM, Life Technologies), as per the manufacturer’s instructions. ..

    Article Title: Electrospun Poly(carbonate-urea-urethane)s Nonwovens with Shape-Memory Properties as a Potential Biomaterial
    Article Snippet: .. The assay was performed using calcein AM (2 μM, C1430 Life technologies) and ethidium homodimer-1, EthD-1 (E1169, 4 μM, Life Technologies), as per the manufacturer’s instructions. ..

    Article Title: Genetically targeted chemical assembly of polymers specifically localized extracellularly to surface membranes of living neurons.
    Article Snippet: To examine cell viability of neurons after reaction, coverslips with live HRP(+) neurons were fixed on the sample platform of a Leica DMi8 microscope and imaged with the YFP signal. .. After polymerization (or fixation in 4% PFA at room temperature for 15 min and permeabilization with 0.03% Triton X-100 at room temperature for 15 min as the positive control), dead cells were stained red with 4 μM EthD-1 from Invitrogen LIVE/DEAD viability/cytotoxicity kit (Invitrogen, L3224) in Tyrode’s solution at room temperature for 30 min and imaged in the red channel. ..

    Article Title: Flexible Organic Photovoltaic-Powered Hydrogel Bioelectronic Dressing With Biomimetic Electrical Stimulation for Healing Infected Diabetic Wounds.
    Article Snippet: .. In addition, the cells were stained with Calcium AM (4 μm) and EthD-1 (2 μm) in PBS for 30 min after their incubation with hydrogels for 3 days, following the instructions provided in the live/dead viability kit (Life Technologies, USA). ..

    Article Title: Hyaluronate- and gelatin-based hydrogels encapsulating doxycycline as a wound dressing for burn injury therapy.
    Article Snippet: Infection is a critical challenge in burn wound therapy.. Wound dressings with antibacterial and multifunctional abilities associated with rapid burn wound healing are urgently needed.. Here, we developed a bioadhesive and injectable ECM-mimicking hydrogel dressing with antibacterial capacity for burn injury therapy, which is crosslinked by dynamic boronate ester bonds between modified hyaluronate and gelatin (HG).

    Article Title: Drug screening on digital microfluidics for cancer precision medicine
    Article Snippet: Recombinant human EGF, recombinant human FGF10, and recombinant human HGF were purchased from Peprotech. .. RBC lysis buffer, EthD-1, erythrocyte lysate, and Cell TrackerTM Green CMFDA Dye were purchased from Invitrogen. .. StemMACS iPS-Brew XF medium was purchased from Miltenyl Biotec (USA).

    Article Title: Acceptor Engineering Produces Ultrafast Nonradiative Decay in NIR-II Aza-BODIPY Nanoparticles for Efficient Osteosarcoma Photothermal Therapy via Concurrent Apoptosis and Pyroptosis
    Article Snippet: .. After incubation for an additional 4 h, 143B cells were washed 3 times with PBS and double staining with Calcein-AM and EthD-1 (Invitrogen) was used to detect live and dead cells. ..

    Article Title: Temperature-Mediated Phase Separation Enables Strong yet Reversible Mechanical and Adhesive Hydrogels.
    Article Snippet: Hydrogels with strong yet reversible mechanical and adhesive properties fabricated in a facile and friendly manner are important for engineering and intelligent electronics applications but are challenging to create and control.. Existing approaches for preparing hydrogels involve complicated pretreatments and produce hydrogels that suffer from limited skin applicability.. Copolymerized hydrogels are expected to present an intriguing target in this field by means of thermoresponsive features, while the perceived intrinsic flaws of brittleness, easy fracture, and weak adhesion enervate the development prospects.

    Positive Control:

    Article Title: Genetically targeted chemical assembly of polymers specifically localized extracellularly to surface membranes of living neurons.
    Article Snippet: To examine cell viability of neurons after reaction, coverslips with live HRP(+) neurons were fixed on the sample platform of a Leica DMi8 microscope and imaged with the YFP signal. .. After polymerization (or fixation in 4% PFA at room temperature for 15 min and permeabilization with 0.03% Triton X-100 at room temperature for 15 min as the positive control), dead cells were stained red with 4 μM EthD-1 from Invitrogen LIVE/DEAD viability/cytotoxicity kit (Invitrogen, L3224) in Tyrode’s solution at room temperature for 30 min and imaged in the red channel. ..

    Staining:

    Article Title: Genetically targeted chemical assembly of polymers specifically localized extracellularly to surface membranes of living neurons.
    Article Snippet: To examine cell viability of neurons after reaction, coverslips with live HRP(+) neurons were fixed on the sample platform of a Leica DMi8 microscope and imaged with the YFP signal. .. After polymerization (or fixation in 4% PFA at room temperature for 15 min and permeabilization with 0.03% Triton X-100 at room temperature for 15 min as the positive control), dead cells were stained red with 4 μM EthD-1 from Invitrogen LIVE/DEAD viability/cytotoxicity kit (Invitrogen, L3224) in Tyrode’s solution at room temperature for 30 min and imaged in the red channel. ..

    Article Title: Flexible Organic Photovoltaic-Powered Hydrogel Bioelectronic Dressing With Biomimetic Electrical Stimulation for Healing Infected Diabetic Wounds.
    Article Snippet: .. In addition, the cells were stained with Calcium AM (4 μm) and EthD-1 (2 μm) in PBS for 30 min after their incubation with hydrogels for 3 days, following the instructions provided in the live/dead viability kit (Life Technologies, USA). ..

    Incubation:

    Article Title: Flexible Organic Photovoltaic-Powered Hydrogel Bioelectronic Dressing With Biomimetic Electrical Stimulation for Healing Infected Diabetic Wounds.
    Article Snippet: .. In addition, the cells were stained with Calcium AM (4 μm) and EthD-1 (2 μm) in PBS for 30 min after their incubation with hydrogels for 3 days, following the instructions provided in the live/dead viability kit (Life Technologies, USA). ..

    Article Title: Hyaluronate- and gelatin-based hydrogels encapsulating doxycycline as a wound dressing for burn injury therapy.
    Article Snippet: Infection is a critical challenge in burn wound therapy.. Wound dressings with antibacterial and multifunctional abilities associated with rapid burn wound healing are urgently needed.. Here, we developed a bioadhesive and injectable ECM-mimicking hydrogel dressing with antibacterial capacity for burn injury therapy, which is crosslinked by dynamic boronate ester bonds between modified hyaluronate and gelatin (HG).

    Article Title: Acceptor Engineering Produces Ultrafast Nonradiative Decay in NIR-II Aza-BODIPY Nanoparticles for Efficient Osteosarcoma Photothermal Therapy via Concurrent Apoptosis and Pyroptosis
    Article Snippet: .. After incubation for an additional 4 h, 143B cells were washed 3 times with PBS and double staining with Calcein-AM and EthD-1 (Invitrogen) was used to detect live and dead cells. ..

    Red Blood Cell Lysis:

    Article Title: Drug screening on digital microfluidics for cancer precision medicine
    Article Snippet: Recombinant human EGF, recombinant human FGF10, and recombinant human HGF were purchased from Peprotech. .. RBC lysis buffer, EthD-1, erythrocyte lysate, and Cell TrackerTM Green CMFDA Dye were purchased from Invitrogen. .. StemMACS iPS-Brew XF medium was purchased from Miltenyl Biotec (USA).

    Double Staining:

    Article Title: Acceptor Engineering Produces Ultrafast Nonradiative Decay in NIR-II Aza-BODIPY Nanoparticles for Efficient Osteosarcoma Photothermal Therapy via Concurrent Apoptosis and Pyroptosis
    Article Snippet: .. After incubation for an additional 4 h, 143B cells were washed 3 times with PBS and double staining with Calcein-AM and EthD-1 (Invitrogen) was used to detect live and dead cells. ..



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    B cells in the IBC tumour microenvironment promote inflammatory responses through high expression of tumour necrosis factor. a Comparison of 6 TNF-related pathway signals in B cells and endothelial cells. b Comparison of 15 in death signalling scores in endothelial cells. c After co-culturing IBC-educated B cell supernatant with endothelial cells, the viability of endothelial cells was assessed by <t>Calcein-AM/EthD-1</t> staining. The experimental schematic is shown. d, f Nec-1 or PTN blocking peptide was used to inhibit endothelial cell death induced by IBC-educated B cell supernatant. The bar graph ( d ) shows the percentage of endothelial cell death, and immunofluorescence imaging ( f ) shows the Calcein-AM + and EthD-1 + endothelial cells. e, g sIn endothelial tube formation assays, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Quantification of tube formation is presented as bar graphs ( e ), and representative tube structures are shown by phase-contrast microscopy ( g ). i TNF + B cells were found around the necrotic endothelial cells based on multiplex immunofluorescence. j Under normal culture, B cell supernatant co-culture, or IBC tumor cell-treated B cell supernatant conditions, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Western blotting was used to detect phosphorylated MLKL. k Under normal culture, B cell supernatant co-culture, or PTN-treated B cell supernatant co-culture conditions, RIPK3 expression in endothelial cells was knocked down or TNF neutralizing antibody was used to block endothelial cell death induced by IBC-educated B cell supernatant. q-PCR was used to measure the expression levels of IL-6 and IL-8 in endothelial cells. l M0 macrophages were cultured under normal conditions, endothelial cell supernatant co-culture, or supernatant of endothelial cells educated with IBC-treated B cells. IgG or IL-6 neutralizing antibody was added, and q-PCR was used to measure the expression of M2 macrophage markers (IL-10, TGF-β, and CD206) in M0 macrophages. m Diagram of the mechanism of interactions between tumour cells, B cells and endothelial cells in the IBC tumour microenvironment
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    B cells in the IBC tumour microenvironment promote inflammatory responses through high expression of tumour necrosis factor. a Comparison of 6 TNF-related pathway signals in B cells and endothelial cells. b Comparison of 15 in death signalling scores in endothelial cells. c After co-culturing IBC-educated B cell supernatant with endothelial cells, the viability of endothelial cells was assessed by <t>Calcein-AM/EthD-1</t> staining. The experimental schematic is shown. d, f Nec-1 or PTN blocking peptide was used to inhibit endothelial cell death induced by IBC-educated B cell supernatant. The bar graph ( d ) shows the percentage of endothelial cell death, and immunofluorescence imaging ( f ) shows the Calcein-AM + and EthD-1 + endothelial cells. e, g sIn endothelial tube formation assays, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Quantification of tube formation is presented as bar graphs ( e ), and representative tube structures are shown by phase-contrast microscopy ( g ). i TNF + B cells were found around the necrotic endothelial cells based on multiplex immunofluorescence. j Under normal culture, B cell supernatant co-culture, or IBC tumor cell-treated B cell supernatant conditions, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Western blotting was used to detect phosphorylated MLKL. k Under normal culture, B cell supernatant co-culture, or PTN-treated B cell supernatant co-culture conditions, RIPK3 expression in endothelial cells was knocked down or TNF neutralizing antibody was used to block endothelial cell death induced by IBC-educated B cell supernatant. q-PCR was used to measure the expression levels of IL-6 and IL-8 in endothelial cells. l M0 macrophages were cultured under normal conditions, endothelial cell supernatant co-culture, or supernatant of endothelial cells educated with IBC-treated B cells. IgG or IL-6 neutralizing antibody was added, and q-PCR was used to measure the expression of M2 macrophage markers (IL-10, TGF-β, and CD206) in M0 macrophages. m Diagram of the mechanism of interactions between tumour cells, B cells and endothelial cells in the IBC tumour microenvironment
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    Image Search Results


    B cells in the IBC tumour microenvironment promote inflammatory responses through high expression of tumour necrosis factor. a Comparison of 6 TNF-related pathway signals in B cells and endothelial cells. b Comparison of 15 in death signalling scores in endothelial cells. c After co-culturing IBC-educated B cell supernatant with endothelial cells, the viability of endothelial cells was assessed by Calcein-AM/EthD-1 staining. The experimental schematic is shown. d, f Nec-1 or PTN blocking peptide was used to inhibit endothelial cell death induced by IBC-educated B cell supernatant. The bar graph ( d ) shows the percentage of endothelial cell death, and immunofluorescence imaging ( f ) shows the Calcein-AM + and EthD-1 + endothelial cells. e, g sIn endothelial tube formation assays, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Quantification of tube formation is presented as bar graphs ( e ), and representative tube structures are shown by phase-contrast microscopy ( g ). i TNF + B cells were found around the necrotic endothelial cells based on multiplex immunofluorescence. j Under normal culture, B cell supernatant co-culture, or IBC tumor cell-treated B cell supernatant conditions, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Western blotting was used to detect phosphorylated MLKL. k Under normal culture, B cell supernatant co-culture, or PTN-treated B cell supernatant co-culture conditions, RIPK3 expression in endothelial cells was knocked down or TNF neutralizing antibody was used to block endothelial cell death induced by IBC-educated B cell supernatant. q-PCR was used to measure the expression levels of IL-6 and IL-8 in endothelial cells. l M0 macrophages were cultured under normal conditions, endothelial cell supernatant co-culture, or supernatant of endothelial cells educated with IBC-treated B cells. IgG or IL-6 neutralizing antibody was added, and q-PCR was used to measure the expression of M2 macrophage markers (IL-10, TGF-β, and CD206) in M0 macrophages. m Diagram of the mechanism of interactions between tumour cells, B cells and endothelial cells in the IBC tumour microenvironment

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Single-cell transcriptome analysis reveals the malignant characteristics of tumour cells and the immunosuppressive landscape in HER2-positive inflammatory breast cancer

    doi: 10.1186/s13046-025-03454-z

    Figure Lengend Snippet: B cells in the IBC tumour microenvironment promote inflammatory responses through high expression of tumour necrosis factor. a Comparison of 6 TNF-related pathway signals in B cells and endothelial cells. b Comparison of 15 in death signalling scores in endothelial cells. c After co-culturing IBC-educated B cell supernatant with endothelial cells, the viability of endothelial cells was assessed by Calcein-AM/EthD-1 staining. The experimental schematic is shown. d, f Nec-1 or PTN blocking peptide was used to inhibit endothelial cell death induced by IBC-educated B cell supernatant. The bar graph ( d ) shows the percentage of endothelial cell death, and immunofluorescence imaging ( f ) shows the Calcein-AM + and EthD-1 + endothelial cells. e, g sIn endothelial tube formation assays, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Quantification of tube formation is presented as bar graphs ( e ), and representative tube structures are shown by phase-contrast microscopy ( g ). i TNF + B cells were found around the necrotic endothelial cells based on multiplex immunofluorescence. j Under normal culture, B cell supernatant co-culture, or IBC tumor cell-treated B cell supernatant conditions, Nec-1 or PTN blocking peptide was used to block endothelial cell death induced by IBC-educated B cell supernatant. Western blotting was used to detect phosphorylated MLKL. k Under normal culture, B cell supernatant co-culture, or PTN-treated B cell supernatant co-culture conditions, RIPK3 expression in endothelial cells was knocked down or TNF neutralizing antibody was used to block endothelial cell death induced by IBC-educated B cell supernatant. q-PCR was used to measure the expression levels of IL-6 and IL-8 in endothelial cells. l M0 macrophages were cultured under normal conditions, endothelial cell supernatant co-culture, or supernatant of endothelial cells educated with IBC-treated B cells. IgG or IL-6 neutralizing antibody was added, and q-PCR was used to measure the expression of M2 macrophage markers (IL-10, TGF-β, and CD206) in M0 macrophages. m Diagram of the mechanism of interactions between tumour cells, B cells and endothelial cells in the IBC tumour microenvironment

    Article Snippet: HUVECs from control and experimental groups were incubated with 2 μM Calcein-AM and 4 μM Ethidium Homodimer-1 (EthD-1) (MCE, HY-D0093) for 10 min in the dark.

    Techniques: Expressing, Comparison, Staining, Blocking Assay, Immunofluorescence, Imaging, Microscopy, Multiplex Assay, Co-Culture Assay, Western Blot, Cell Culture